Cellosaurus PC3-eGFP-Neo/Fluc-Puro (CVCL_D5K9)
Cell line name | PC3-eGFP-Neo/Fluc-Puro |
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Accession | CVCL_D5K9 |
Resource Identification Initiative | To cite this cell line use: PC3-eGFP-Neo/Fluc-Puro (RRID:CVCL_D5K9) |
Comments | Population: Caucasian. Characteristics: Reporter cell line, transfected with both the LV-eGFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-eGFP-P2A-Neo encodes for eGFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter and LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL125). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: Metastatic; Bone; UBERON=UBERON_0002481. |
Sequence variations |
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Disease | Prostate carcinoma (NCIt: C4863) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0035 (PC-3) |
Sex of cell | Male |
Age at sampling | 62Y |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Imanis; CL125 |
Encyclopedic resources | Wikidata; Q127383020 |
Entry history | |
Entry creation | 02-May-2024 |
Last entry update | 10-Sep-2024 |
Version number | 2 |