ID   PC3-eGFP-Neo/Fluc-Puro
AC   CVCL_D5K9
DR   Imanis; CL125
DR   Wikidata; Q127383020
CC   Population: Caucasian.
CC   Characteristics: Reporter cell line, transfected with both the LV-eGFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-eGFP-P2A-Neo encodes for eGFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter and LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL125).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Lys139Argfs*31 (c.414delC) (413delC); ClinVar=VCV000133281; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: Metastatic; Bone; UBERON=UBERON_0002481.
DI   NCIt; C4863; Prostate carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0035 ! PC-3
SX   Male
AG   62Y
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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