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Cellosaurus Jurkat 2H4 (CVCL_D5JP)

[Text version]
Cell line name Jurkat 2H4
Synonyms 2H4; #2H4
Accession CVCL_D5JP
Resource Identification Initiative To cite this cell line use: Jurkat 2H4 (RRID:CVCL_D5JP)
Comments Population: Caucasian.
Characteristics: Part of an immunotoxicity assay system, the MultiImmunoTox Assay (MITA) (PubMed=24603311).
Characteristics: Transfected with three constructs, one expressing green-light luciferase (SLG) under the control of an IL2 promoter, one expressing orange-light luciferase (SLO) under the control of an IFNG promoter and the third expressing red-light luciferase (SLR) under the control of a GAPDH promoter (PubMed=21569788).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U7; Phrixothrix hirtus red luciferase (Note=SLR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=SLG).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=With p.Thr226Asn = SLO, orange).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0065 (Jurkat)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): TKG=TKG 0806

Markers:
AmelogeninX,Y
CSF1PO11,13
D5S8189
D7S8208,10
D13S3178,11
D16S53911
TH016,9.3
TPOX8,10
vWA18

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Publications

PubMed=21569788; DOI=10.1016/j.taap.2011.04.017
Saito R., Hirakawa S., Ohara H., Yasuda M., Yamazaki T., Nishii S., Aiba S.
Nickel differentially regulates NFAT and NF-kappaB activation in T cell signaling.
Toxicol. Appl. Pharmacol. 254:245-255(2011)

PubMed=24603311; DOI=10.1016/j.tiv.2014.02.013
Kimura Y., Fujimura C., Ito Y., Takahashi T., Aiba S.
Evaluation of the Multi-ImmunoTox assay composed of 3 human cytokine reporter cells by examining immunological effects of drugs.
Toxicol. In Vitro 28:759-768(2014)

PubMed=29594315; DOI=10.1007/s00204-018-2199-7
Kimura Y., Fujimura C., Ito Y., Takahashi T., Terui H., Aiba S.
Profiling the immunotoxicity of chemicals based on in vitro evaluation by a combination of the Multi-ImmunoTox assay and the IL-8 Luc assay.
Arch. Toxicol. 92:2043-2054(2018)

Cross-references
Cell line collections (Providers) TKG; TKG 0806
Encyclopedic resources Wikidata; Q127382402
Entry history
Entry creation02-May-2024
Last entry update19-Dec-2024
Version number3