ID   Jurkat 2H4
AC   CVCL_D5JP
SY   2H4; #2H4
DR   TKG; TKG 0806
DR   Wikidata; Q127382402
RX   PubMed=21569788;
RX   PubMed=24603311;
RX   PubMed=29594315;
CC   Population: Caucasian.
CC   Characteristics: Part of an immunotoxicity assay system, the MultiImmunoTox Assay (MITA) (PubMed=24603311).
CC   Characteristics: Transfected with three constructs, one expressing green-light luciferase (SLG) under the control of an IL2 promoter, one expressing orange-light luciferase (SLO) under the control of an IFNG promoter and the third expressing red-light luciferase (SLR) under the control of a GAPDH promoter (PubMed=21569788).
CC   Sequence variation: Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U7; Phrixothrix hirtus red luciferase (Note=SLR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=SLG).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=With p.Thr226Asn = SLO, orange).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
ST   Source(s): TKG=TKG 0806
ST   Amelogenin: X,Y
ST   CSF1PO: 11,13
ST   D13S317: 8,11
ST   D16S539: 11
ST   D5S818: 9
ST   D7S820: 8,10
ST   TH01: 6,9.3
ST   TPOX: 8,10
ST   vWA: 18
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0065 ! Jurkat
SX   Male
AG   14Y
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
//
RX   PubMed=21569788; DOI=10.1016/j.taap.2011.04.017;
RA   Saito R., Hirakawa S., Ohara H., Yasuda M., Yamazaki T., Nishii S.,
RA   Aiba S.;
RT   "Nickel differentially regulates NFAT and NF-kappaB activation in T
RT   cell signaling.";
RL   Toxicol. Appl. Pharmacol. 254:245-255(2011).
//
RX   PubMed=24603311; DOI=10.1016/j.tiv.2014.02.013;
RA   Kimura Y., Fujimura C., Ito Y., Takahashi T., Aiba S.;
RT   "Evaluation of the Multi-ImmunoTox assay composed of 3 human cytokine
RT   reporter cells by examining immunological effects of drugs.";
RL   Toxicol. In Vitro 28:759-768(2014).
//
RX   PubMed=29594315; DOI=10.1007/s00204-018-2199-7;
RA   Kimura Y., Fujimura C., Ito Y., Takahashi T., Terui H., Aiba S.;
RT   "Profiling the immunotoxicity of chemicals based on in vitro
RT   evaluation by a combination of the Multi-ImmunoTox assay and the IL-8
RT   Luc assay.";
RL   Arch. Toxicol. 92:2043-2054(2018).
//