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Cellosaurus THP-G8 (CVCL_D5JK)

[Text version]
Cell line name THP-G8
Accession CVCL_D5JK
Resource Identification Initiative To cite this cell line use: THP-G8 (RRID:CVCL_D5JK)
Comments Population: Japanese.
Characteristics: Used for the interleukin-8 reporter gene assay (IL-8 Luc assay) (PubMed=21920952).
Characteristics: Part of an immunotoxicity assay system, the MultiImmunoTox Assay (MITA) (PubMed=24603311).
Characteristics: Transfected with two constructs, one expressing orange-light luciferase (SLO) under the control of an IL8 promoter, the other expressing red-light luciferase (SLR) under the control of a GAPDH promoter (PubMed=21920952).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U7; Phrixothrix hirtus red luciferase (Note=SLR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=With p.Thr226Asn = SLO, orange).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
  • Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
Disease Childhood acute monocytic leukemia (NCIt: C9163)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0006 (THP-1)
Sex of cell Male
Age at sampling 1Y
Category Cancer cell line
STR profile Source(s): TKG=TKG 0809

Markers:
AmelogeninX,Y
CSF1PO11,13
D5S81811,12
D7S82010
D13S31713
D16S53911,12
TH018,9.3
TPOX8,11
vWA16

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Web pages https://read.oecd-ilibrary.org/environment/test-no-442e-in-vitro-skin-sensitisation_9789264264359-en
Publications

PubMed=21920952; DOI=10.1093/toxsci/kfr237
Takahashi T., Kimura Y., Saito R., Nakajima Y., Ohmiya Y., Yamasaki K., Aiba S.
An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8.
Toxicol. Sci. 124:359-369(2011)

PubMed=24603311; DOI=10.1016/j.tiv.2014.02.013
Kimura Y., Fujimura C., Ito Y., Takahashi T., Aiba S.
Evaluation of the Multi-ImmunoTox assay composed of 3 human cytokine reporter cells by examining immunological effects of drugs.
Toxicol. In Vitro 28:759-768(2014)

PubMed=29594315; DOI=10.1007/s00204-018-2199-7
Kimura Y., Fujimura C., Ito Y., Takahashi T., Terui H., Aiba S.
Profiling the immunotoxicity of chemicals based on in vitro evaluation by a combination of the Multi-ImmunoTox assay and the IL-8 Luc assay.
Arch. Toxicol. 92:2043-2054(2018)

Cross-references
Cell line collections (Providers) TKG; TKG 0809
Encyclopedic resources Wikidata; Q127384395
Entry history
Entry creation02-May-2024
Last entry update19-Dec-2024
Version number3