ID   THP-G8
AC   CVCL_D5JK
DR   TKG; TKG 0809
DR   Wikidata; Q127384395
RX   PubMed=21920952;
RX   PubMed=24603311;
RX   PubMed=29594315;
WW   Info; OECD; -; https://read.oecd-ilibrary.org/environment/test-no-442e-in-vitro-skin-sensitisation_9789264264359-en
CC   Population: Japanese.
CC   Characteristics: Used for the interleukin-8 reporter gene assay (IL-8 Luc assay) (PubMed=21920952).
CC   Characteristics: Part of an immunotoxicity assay system, the MultiImmunoTox Assay (MITA) (PubMed=24603311).
CC   Characteristics: Transfected with two constructs, one expressing orange-light luciferase (SLO) under the control of an IL8 promoter, the other expressing red-light luciferase (SLR) under the control of a GAPDH promoter (PubMed=21920952).
CC   Sequence variation: Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
CC   Sequence variation: Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U8; Phrixothrix hirtus green luciferase (Note=With p.Thr226Asn = SLO, orange).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9U4U7; Phrixothrix hirtus red luciferase (Note=SLR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
ST   Source(s): TKG=TKG 0809
ST   Amelogenin: X,Y
ST   CSF1PO: 11,13
ST   D13S317: 13
ST   D16S539: 11,12
ST   D5S818: 11,12
ST   D7S820: 10
ST   TH01: 8,9.3
ST   TPOX: 8,11
ST   vWA: 16
DI   NCIt; C9163; Childhood acute monocytic leukemia
DI   ORDO; Orphanet_514; Acute monoblastic/monocytic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0006 ! THP-1
SX   Male
AG   1Y
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 14-08-25; Version: 5
//
RX   PubMed=24603311; DOI=10.1016/j.tiv.2014.02.013;
RA   Kimura, Yutaka
RA   Fujimura, Chizu
RA   Ito, Yumiko
RA   Takahashi, Toshiya
RA   Aiba, Setsuya
RT   "Evaluation of the Multi-ImmunoTox assay composed of 3 human cytokine
RT   reporter cells by examining immunological effects of drugs.";
RL   Toxicol. In Vitro 28:759-768(2014).
//
RX   PubMed=29594315; DOI=10.1007/s00204-018-2199-7;
RA   Kimura, Yutaka
RA   Fujimura, Chizu
RA   Ito, Yumiko
RA   Takahashi, Toshiya
RA   Terui, Hitoshi
RA   Aiba, Setsuya
RT   "Profiling the immunotoxicity of chemicals based on in vitro
RT   evaluation by a combination of the Multi-ImmunoTox assay and the IL-8
RT   Luc assay.";
RL   Arch. Toxicol. 92:2043-2054(2018).
//
RX   PubMed=21920952; DOI=10.1093/toxsci/kfr237;
RA   Takahashi, Toshiya
RA   Kimura, Yutaka
RA   Saito, Rumiko
RA   Nakajima, Yoshihiro
RA   Ohmiya, Yoshihiro
RA   Yamasaki, Kenshi
RA   Aiba, Setsuya
RT   "An in vitro test to screen skin sensitizers using a stable
RT   THP-1-derived IL-8 reporter cell line, THP-G8.";
RL   Toxicol. Sci. 124:359-369(2011).
//