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Cellosaurus HEK293 Flp-In T-Rex PINK1 KO clone F1 (CVCL_D5JH)

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Cell line name HEK293 Flp-In T-Rex PINK1 KO clone F1
Synonyms Flp-In T-Rex HEK293 PINK1 CRISPR/Cas9 knock-out
Accession CVCL_D5JH
Resource Identification Initiative To cite this cell line use: HEK293 Flp-In T-Rex PINK1 KO clone F1 (RRID:CVCL_D5JH)
Comments Knockout cell: Method=CRISPR/Cas9; HGNC; 14581; PINK1.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_U427 (Flp-In-T-REx-293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=35042401; DOI=10.1098/rsob.210264; PMCID=PMC8767193
Kakade P.S., Ojha H., Raimi O.G., Shaw A., Waddell A.D., Ault J.R., Burel S., Brockmann K., Kumar A., Ahangar M.S., Krysztofinska E.M., Macartney T., Bayliss R., Fitzgerald J.C., Muqit M.M.K.
Mapping of a N-terminal alpha-helix domain required for human PINK1 stabilization, serine228 autophosphorylation and activation in cells.
Open Biol. 12:210264.1-210264.16(2022)

Cross-references
Encyclopedic resources Wikidata; Q127381640
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2