ID   HEK293 Flp-In T-Rex PINK1 KO clone F1
AC   CVCL_D5JH
SY   Flp-In T-Rex HEK293 PINK1 CRISPR/Cas9 knock-out
DR   Wikidata; Q127381640
RX   PubMed=35042401;
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 14581; PINK1.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_U427 ! Flp-In-T-REx-293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=35042401; DOI=10.1098/rsob.210264; PMCID=PMC8767193;
RA   Kakade P.S., Ojha H., Raimi O.G., Shaw A., Waddell A.D., Ault J.R.,
RA   Burel S., Brockmann K., Kumar A., Ahangar M.S., Krysztofinska E.M.,
RA   Macartney T., Bayliss R., Fitzgerald J.C., Muqit M.M.K.;
RT   "Mapping of a N-terminal alpha-helix domain required for human PINK1
RT   stabilization, serine228 autophosphorylation and activation in
RT   cells.";
RL   Open Biol. 12:210264.1-210264.16(2022).
//