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Cellosaurus HeLa S3 RUBCNL KO/HA-PRKN/HaloTag7-mGFP (CVCL_D5AL)

[Text version]
Cell line name HeLa S3 RUBCNL KO/HA-PRKN/HaloTag7-mGFP
Synonyms Pacer KO S3 HeLa + HA-Parkin/pSu9 + HaloTag7-mGFP
Accession CVCL_D5AL
Resource Identification Initiative To cite this cell line use: HeLa S3 RUBCNL KO/HA-PRKN/HaloTag7-mGFP (RRID:CVCL_D5AL)
Comments Population: African American.
Knockout cell: Method=CRISPR/Cas9; HGNC; 20420; RUBCNL.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 8607; PRKN (Note=With a N-terminal HA tag).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D5AH (HeLa S3 RUBCNL KO clone 17)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

DOI=10.1101/2023.08.28.555228
Tudorica D.A., Basak B., Cordova A.P., Khuu G., Rose K., Lazarou M., Holzbaur E.L.F., Hurley J.H.
A RAB7A phosphoswitch coordinates rubicon homology protein regulation of PINK1/parkin dependent mitophagy.
bioRxiv 2023:08.28.555228-08.28.555228(2023)

Cross-references
Encyclopedic resources Wikidata; Q127381697
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2