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Cellosaurus U2OS SP2 CL4 (CVCL_D4Y0)

[Text version]
Cell line name U2OS SP2 CL4
Accession CVCL_D4Y0
Resource Identification Initiative To cite this cell line use: U2OS SP2 CL4 (RRID:CVCL_D4Y0)
Comments Population: Caucasian.
Characteristics: Transfected with two constructs, one containing the PEX3 peroxisome targeting sequence (PTS) fused to RFP and FKBP (PTS-RFP-FKBP) and the other to the FKBP-rapamycin-binding (FRB) domain of mTOR linked to the N-terminal half of mouse BicD2 (BicD2N) and GFP (GFP-BicD2N-FRB) (PubMed=32436764).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3711; FKBP1A.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3942; MTOR (Note=With p.Met1_Arg2018del and p.Gln2114_Trp2549del = FRB).
Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1924145; Bicd2 (Note=With p.Arg401_Leu820del = Bicd2N).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=32436764; DOI=10.1177/2472555220920581; PMCID=PMC7116108
Vincent J., Preston M., Mouchet E., Laugier N., Corrigan A., Boulanger J., Brown D.G., Clark R., Wigglesworth M., Carter A.P., Bullock S.L.
A high-throughput cellular screening assay for small-molecule inhibitors and activators of cytoplasmic dynein-1-based cargo transport.
SLAS Discov. 25:985-999(2020)

Cross-references
Encyclopedic resources Wikidata; Q127384441
Entry history
Entry creation02-May-2024
Last entry update19-Dec-2024
Version number3