Cellosaurus cell line HepG2-HSP (CVCL

Cellosaurus HepG2-HSP (CVCL_D3XE)

[Text version]

Cell line name

HepG2-HSP

Accession

CVCL_D3XE

Resource Identification Initiative

To cite this cell line use: HepG2-HSP (RRID:CVCL_D3XE)

Comments

Population: Caucasian.
Characteristics: Transduced with a pQMSCV-pHSP70B-TRE-pCMVmin-EGFP-IRES-tTA construct. The heat-activated HSPA6/HSP70B' promoter drives bicistronic gene expression of EGFP and of the tetracycline-controlled transactivator (tTA), which is mediated by an internal ribosomal entry site (IRES). Then, the tTA protein binds to TRE and activates the cytomegalovirus minimum promoter (pCMVmin) upstream of EGFP, which in turn induces further expression of the EGFP and tTA genes, providing a transcriptional positive feedback loop (PubMed=30962099).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.

Sequence variations

Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).

Disease

Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0027 (Hep-G2)
Children:

CVCL_D3XF (HepG2/8F_HS)

Sex of cell

Male

Age at sampling

15Y