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Cellosaurus G1E (CVCL_D046)

[Text version]
Cell line name G1E
Synonyms GATA-1(-) Erythroid
Accession CVCL_D046
Resource Identification Initiative To cite this cell line use: G1E (RRID:CVCL_D046)
Comments Part of: ENCODE project mouse cell lines.
Characteristics: Proliferates continuously in culture as immature erythroblasts and undergo terminal maturation when Gata1 function is restored.
Characteristics: EPO and KITLG dependent.
Doubling time: ~12 hours (ENCODE=ENCBS159ZTO).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:990; BCL2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Breed/subspecies: 129S/SvEv-Gpi1<c>.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_WI94 (GATA-1- ES cell clone 74)
Children:
CVCL_D4C9 (G1E-ER2)CVCL_D047 (G1E-ER4)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/G1E_Hardison_protocol.pdf
Publications

PubMed=9032291; DOI=10.1128/mcb.17.3.1642; PMCID=PMC231889
Weiss M.J., Yu C., Orkin S.H.
Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line.
Mol. Cell. Biol. 17:1642-1651(1997)

PubMed=10381501; DOI=10.1182/blood.V94.1.87.413k41_87_96
Gregory T., Yu C., Ma A., Orkin S.H., Blobel G.A., Weiss M.J.
GATA-1 and erythropoietin cooperate to promote erythroid cell survival by regulating bcl-xL expression.
Blood 94:87-96(1999)

PubMed=12832487; DOI=10.1128/mcb.23.14.5031-5042.2003; PMCID=PMC162202
Rylski M., Welch J.J., Chen Y.-Y., Letting D.L., Diehl J.A., Chodosh L.A., Blobel G.A., Weiss M.J.
GATA-1-mediated proliferation arrest during erythroid maturation.
Mol. Cell. Biol. 23:5031-5042(2003)

PubMed=25729644; DOI=10.1016/j.gdata.2015.01.008; PMCID=PMC4338950
Jain D., Mishra T., Giardine B.M., Keller C.A., Morrissey C.S., Magargee S.P., Dorman C.M., Long M., Weiss M.J., Hardison R.C.
Dynamics of GATA1 binding and expression response in a GATA1-induced erythroid differentiation system.
Genom. Data 4:1-7(2015)

Cross-references
Biological sample resources ENCODE; ENCBS159ZTO
ENCODE; ENCBS324ENC
ENCODE; ENCBS431QXW
ENCODE; ENCBS503ARZ
ENCODE; ENCBS566SQH
ENCODE; ENCBS602TAQ
ENCODE; ENCBS807UWB
Encyclopedic resources Wikidata; Q54835375
Experimental variables resources EFO; EFO_0002034
Entry history
Entry creation22-Oct-2012
Last entry update19-Dec-2024
Version number17