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Cellosaurus NCI-H1299/Cas9 AAVS1 (CVCL_C9BI)

[Text version]
Cell line name NCI-H1299/Cas9 AAVS1
Synonyms NCI-H1299-Cas9 AAVS1
Accession CVCL_C9BI
Resource Identification Initiative To cite this cell line use: NCI-H1299/Cas9 AAVS1 (RRID:CVCL_C9BI)
Comments Population: Caucasian.
Characteristics: Stably expresses a CMV-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-PuroR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL501).
Karyotypic information: Has lost chromosome Y (from parent cell line).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Lung large cell carcinoma (NCIt: C4450)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0060 (NCI-H1299)
Sex of cell Male
Age at sampling 43Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL501
Encyclopedic resources Wikidata; Q123033152
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4