Cellosaurus Sufu-KO-LIGHT (CVCL_C8QR)
| Cell line name | Sufu-KO-LIGHT |
|---|---|
| Accession | CVCL_C8QR |
| Resource Identification Initiative | To cite this cell line use: Sufu-KO-LIGHT (RRID:CVCL_C8QR) |
| Comments | Characteristics: Used to discover small-molecule antagonists of Gli function. Exhibits constitutive Hh pathway activity driven by endogenous Gli factors (PubMed=32268014). Characteristics: Transfected with a firefly luciferase reporter driven by eight Gli binding sites and a delta-crystallin basal promoter (8XGHBS-FL) and with a SV40 promoter-driven Renilla luciferase reporter (PubMed=32268014). Knockout cell: Method=KO mouse; MGI; MGI:1345643; Sufu. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P27652; Renilla-type luciferase. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) |
| Sex of cell | Female |
| Age at sampling | Embryo |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=32268014; DOI=10.1002/cmdc.202000169; PMCID=PMC7311267 |
| Cross-references | |
| Cell line databases/resources | CLO; CLO_0037222 |
| Encyclopedic resources | Wikidata; Q123033561 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 10-Sep-2024 |
| Version number | 3 |