ID   Sufu-KO-LIGHT
AC   CVCL_C8QR
DR   CLO; CLO_0037222
DR   Wikidata; Q123033561
RX   PubMed=32268014;
CC   Characteristics: Used to discover small-molecule antagonists of Gli function. Exhibits constitutive Hh pathway activity driven by endogenous Gli factors (PubMed=32268014).
CC   Characteristics: Transfected with a firefly luciferase reporter driven by eight Gli binding sites and a delta-crystallin basal promoter (8XGHBS-FL) and with a SV40 promoter-driven Renilla luciferase reporter (PubMed=32268014).
CC   Knockout cell: Method=KO mouse; MGI; MGI:1345643; Sufu.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Female
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
//
RX   PubMed=32268014; DOI=10.1002/cmdc.202000169; PMCID=PMC7311267;
RA   Hom M.E., Ondrus A.E., Sakata-Kato T., Rack P.G., Chen J.K.;
RT   "Bicyclic imidazolium inhibitors of Gli transcription factor
RT   activity.";
RL   ChemMedChem 15:1044-1049(2020).
//