Cellosaurus HeLa HaloTag-LC3/ATG3-K62D/K64D-mCherry (CVCL_C8QM)
| Cell line name | HeLa HaloTag-LC3/ATG3-K62D/K64D-mCherry |
|---|---|
| Accession | CVCL_C8QM |
| Resource Identification Initiative | To cite this cell line use: HeLa HaloTag-LC3/ATG3-K62D/K64D-mCherry (RRID:CVCL_C8QM) |
| Comments | Population: African American. Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux. Also expresses an internally mCherry-tagged ATG3 with p.Lys62Asp and p.Lys64Asp. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20962; ATG3. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:20962; ATG3 (Note=With p.Lys62Asp, p.Lys64Asp and a mCherry reporter inserted between Glu-125 and Ile-126). Genetic integration: Method=Transduction; Gene=RGD; 621315; Map1lc3b. Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Caution: While described in the preprint DOI=10.1101/2023.07.17.549391, this cell line is no longer used as it has been found not be effective (personal communication of Ren, Xuefeng). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_C8QC (HeLa HaloTag-LC3) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Publications | DOI=10.1101/2023.07.17.549391 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q123031612 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 19-Dec-2024 |
| Version number | 5 |