Cellosaurus cell line HeLa HaloTag-LC3 (CVCL

Cellosaurus HeLa HaloTag-LC3 (CVCL_C8QC)

[Text version]

Cell line name

HeLa HaloTag-LC3

Accession

CVCL_C8QC

Resource Identification Initiative

To cite this cell line use: HeLa HaloTag-LC3 (RRID:CVCL_C8QC)

Comments

Population: African American.
Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20962; ATG3.
Genetic integration: Method=Transduction; Gene=RGD; 621315; Map1lc3b.
Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Caution: While described in the preprint DOI=10.1101/2023.07.17.549391, this cell line is no longer used as it has been found not be effective (personal communication of Ren, Xuefeng).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.

Disease

Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_C7IT (HeLa S3 ATG3 KO)
Children:

CVCL_C8QE (HeLa HaloTag-LC3/ATG3-C264A-mCherry)	CVCL_C8QI (HeLa HaloTag-LC3/ATG3-H262A-mCherry)	CVCL_C8QK (HeLa HaloTag-LC3/ATG3-H266A-mCherry)
CVCL_C8QL (HeLa HaloTag-LC3/ATG3-K208D-mCherry)	CVCL_C8QM (HeLa HaloTag-LC3/ATG3-K62D/K64D-mCherry)	CVCL_C8QD (HeLa HaloTag-LC3/ATG3-mCherry)
CVCL_C8QJ (HeLa HaloTag-LC3/ATG3-R265A-mCherry)	CVCL_C8QH (HeLa HaloTag-LC3/ATG3-T244A-mCherry)	CVCL_C8QF (HeLa HaloTag-LC3/ATG3-Y209A-mCherry)
CVCL_C8QG (HeLa HaloTag-LC3/ATG3-Y210A-mCherry)

Sex of cell

Female

Age at sampling

30Y6M