Cellosaurus cell line KOLF2.1J TH-TdTomato (CVCL

Cross-references
Cell line name	KOLF2.1J TH-TdTomato
Synonyms	TH-TdTomato KOLF2.1J Reporter line
Accession	CVCL_C8FQ
Resource Identification Initiative	To cite this cell line use: KOLF2.1J TH-TdTomato (RRID:CVCL_C8FQ)
Comments	Population: Caucasian; British. Characteristics: Fluorescent (tdTomato) reporter cell line for TH expression. Using CRISPR/Cas9 a P2A-tdTomato construct was introduced in the C-terminal end of TH. Genetic integration: Method=Transfection/transduction; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Miscellaneous: Cell line information from personal communication of Coccia, Elena. Derived from site: In situ; Skin; UBERON=UBERON_0002097. Cell type: Fibroblast of skin; CL=CL_0002620.
Sequence variations	Mutation; HGNC; 18037; ARID2; Simple_corrected; p.Pro197Hisfs*12 (c.590_608delCTAAAATCATCACTTTACT); Zygosity=Heterozygous; Note=By CRISPR/Cas9 (from parent cell line). Mutation; HGNC; 2201; COL3A1; Simple; c.3526-1G>A; Zygosity=Heterozygous; Note=Splice acceptor mutation (from parent cell line). Mutation; HGNC; 2201; COL3A1; Simple; p.Gly1176Ser (c.3526G>A); Zygosity=Heterozygous (from parent cell line).
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_B5P3 (KOLF2.1J)
Sex of cell	Male
Age at sampling	55-59Y
Category	Induced pluripotent stem cell
Encyclopedic resources	Wikidata; Q123032843
Entry history
Entry creation	29-Jun-2023
Last entry update	10-Sep-2024
Version number	3