Cellosaurus WAe009-A-H (CVCL_C7FS)
| Cell line name | WAe009-A-H |
|---|---|
| Synonyms | H9-NKX2.5GFP/TBX5Td-T2A-18; SKLRMe004-A |
| Accession | CVCL_C7FS |
| Resource Identification Initiative | To cite this cell line use: WAe009-A-H (RRID:CVCL_C7FS) |
| Comments | From: State Key Laboratory of Reproductive Medicine, Nanjing Medical University; Nanjing; China. Characteristics: Using CRISPR/Cas9 two constructs T2A-EGFP-PGKp-PuroR and T2A-Tdtomato-EF1a-Neo were respectively inserted at the C-terminus of NKX2.5 and TBX5 (PubMed=36608451). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_9773 (WA09) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=36608451; DOI=10.1016/j.scr.2023.103015 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; WAe009-A-H |
| Encyclopedic resources | Wikidata; Q117704873 |
| Entry history | |
| Entry creation | 21-Mar-2023 |
| Last entry update | 10-Sep-2024 |
| Version number | 4 |