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Cellosaurus WAe009-A-99 (CVCL_C6TU)

[Text version]
Cell line name WAe009-A-99
Synonyms dCas9-p300 H9 21; dCas9-p300 H9 clone 21; KITe010-A-99
Accession CVCL_C6TU
Resource Identification Initiative To cite this cell line use: WAe009-A-99 (RRID:CVCL_C6TU)
Comments From: Korea Institute of Toxicology; Daejeon; South Korea.
Characteristics: Transduced with a dCas9-p300-P2A-PuroR construct where the dCas9 is fused to the catalytic HAT core domain of p300 (PubMed=36493708).
Genetic integration: Method=Transduction; Gene=HGNC; 3373; EP300 (Note=With p.Met1_Lys1047del and p.Arg1665_His2414del = catalytic domain).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=36493708; DOI=10.1016/j.scr.2022.102986
Kang E.-H., Kim J.-W., Noh H., Park S.-H., Park H.-J., Yoon S., Kim H.
Establishment of a human embryonic stem cell line, WAe009-A-99, with constitutive expression of the dCas9-p300 fusion protein.
Stem Cell Res. 66:102986-102986(2023)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-99
Biological sample resources BioSamples; SAMEA111365973
Encyclopedic resources Wikidata; Q117704976
Entry history
Entry creation21-Mar-2023
Last entry update10-Sep-2024
Version number4