Cellosaurus CRMi003-A-3 (CVCL_C1P1)
| Cell line name | CRMi003-A-3 |
|---|---|
| Synonyms | Proliving; GMNN-mScarletI Reporter; LUMCi041-A-3 |
| Accession | CVCL_C1P1 |
| Resource Identification Initiative | To cite this cell line use: CRMi003-A-3 (RRID:CVCL_C1P1) |
| Comments | Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=36702081). Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 a mScarletI-GMNN(1-110) construct was introduced in the AAVS1 safe harbor locus (PubMed=36702081). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 6VVTK; mScarlet-I (Note=Red fluorescent protein). Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_B5S5 (CRMi003-A-2) |
| Sex of cell | Male |
| Age at sampling | <1D |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=36702081; DOI=10.1016/j.scr.2023.103031 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; CRMi003-A-3 |
| Biological sample resources | BioSamples; SAMEA110425584 |
| Encyclopedic resources | Wikidata; Q114311268 |
| Entry history | |
| Entry creation | 22-Sep-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |