Cellosaurus CRMi003-A-2 (CVCL_B5S5)
| Cell line name | CRMi003-A-2 | |
|---|---|---|
| Synonyms | LUMCi041-A-2; DOXiH2BmTurq2 | |
| Accession | CVCL_B5S5 | |
| Resource Identification Initiative | To cite this cell line use: CRMi003-A-2 (RRID:CVCL_B5S5) | |
| Comments | Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=34775202). Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (PubMed=34775202). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. | |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | |
| Hierarchy | Parent: CVCL_1E71 (NCRM-1) Children:
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| Sex of cell | Male | |
| Age at sampling | <1D | |
| Category | Induced pluripotent stem cell | |
| Publications | PubMed=34775202; DOI=10.1016/j.scr.2021.102592 | |
| Cross-references | ||
| Cell line databases/resources | hPSCreg; CRMi003-A-2 | |
| Biological sample resources | BioSamples; SAMEA10199970 | |
| Encyclopedic resources | Wikidata; Q111733136 | |
| Entry history | ||
| Entry creation | 17-Mar-2022 | |
| Last entry update | 10-Sep-2024 | |
| Version number | 5 | |