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Cellosaurus H9EOS (CVCL_C0VX)

[Text version]
Cell line name H9EOS
Accession CVCL_C0VX
Resource Identification Initiative To cite this cell line use: H9EOS (RRID:CVCL_C0VX)
Comments Characteristics: Transduced with a EOS-C(3+)-GFP/puroR construct. EOS (ETn, Oct-4 and Sox2) combines a highly active promoter from a mouse early transposon (ETn) that is specific for undifferentiated pluripotent stem cells and combined it with Oct-4 (Pou5f1) and Sox2-binding motifs. EOS regulates an enhanced green fluorescent protein (eGFP), an internal ribosome entry site (IRES) and a puromycin resistance gene to allow live-cell visualization and antibiotic selection simultaneously.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=25215486; DOI=10.1016/j.cell.2014.08.029; PMCID=PMC4162745
Takashima Y., Guo G., Loos R., Nichols J., Ficz G., Krueger F., Oxley D., Santos F., Clarke J., Mansfield W., Reik W., Bertone P., Smith A.G.
Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Cell 158:1254-1269(2014)

PubMed=28765214; DOI=10.1242/dev.146811; PMCID=PMC5560041
Guo G., von Meyenn F., Rostovskaya M., Clarke J., Dietmann S., Baker D.E.C., Sahakyan A., Myers S., Bertone P., Reik W., Plath K., Smith A.G.
Epigenetic resetting of human pluripotency.
Development 144:2748-2763(2017)

Cross-references
Encyclopedic resources Wikidata; Q114311558
Entry history
Entry creation22-Sep-2022
Last entry update10-Sep-2024
Version number5