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Cellosaurus 293EL-APP-/- (CVCL_C0I8)

[Text version]
Cell line name 293EL-APP-/-
Accession CVCL_C0I8
Resource Identification Initiative To cite this cell line use: 293EL-APP-/- (RRID:CVCL_C0I8)
Comments Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:620; APP.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3185; EEA1 (Note=With a N-terminal FLAG tag).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C0I7 (293EL)
Children:
CVCL_C0I9 (293EL-APP*)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=36245040; DOI=10.1038/s41467-022-33881-x; PMCID=PMC9573879
Park H., Hundley F.V., Yu Q., Overmyer K.A., Brademan D.R., Serrano L., Paulo J.A., Paoli J.C., Swarup S., Coon J.J., Gygi S.P., Harper J.W.
Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics.
Nat. Commun. 13:6112.1-6112.21(2022)

Cross-references
Encyclopedic resources Wikidata; Q112926923
Entry history
Entry creation23-Jun-2022
Last entry update19-Dec-2024
Version number7