Cellosaurus 10-4A(BFP) (CVCL_B7UX)
| Cell line name | 10-4A(BFP) |
|---|---|
| Synonyms | 10-4ABFP |
| Accession | CVCL_B7UX |
| Resource Identification Initiative | To cite this cell line use: 10-4A(BFP) (RRID:CVCL_B7UX) |
| Comments | Characteristics: Using CRISPR/Cas9 a T2A-TagBFP-NLS construct was introduced at the C-terminus of Acta2 (PubMed=33162897). Characteristics: Transduced with a lentiviral library consisting of 33 genes, some of which are retained in the immortalized cell line (from parent cell line). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5361; ID2. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5362; ID3. Genetic integration: Method=Transduction; Gene=MGI; MGI:1919200; Nanog. Genetic integration: Method=Transduction; Gene=MGI; MGI:99187; Zfp42. Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7. Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen. Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Lung; UBERON=UBERON_0002048. Cell type: Alveolar type 2 fibroblast; CL=CL_4028006. |
| Species of origin | Rattus norvegicus (Rat)
(NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS:0000681. |
| Hierarchy | Parent: CVCL_B7UW (10-4A) |
| Sex of cell | Male |
| Age at sampling | 12-14W |
| Category | Transformed cell line |
| Publications | PubMed=33162897; DOI=10.3389/fphys.2020.567675; PMCID=PMC7582034 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q112926881 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 19-Dec-2024 |
| Version number | 7 |