ID   10-4A(BFP)
AC   CVCL_B7UX
SY   10-4ABFP
DR   Wikidata; Q112926881
RX   PubMed=33162897;
CC   Characteristics: Using CRISPR/Cas9 a T2A-TagBFP-NLS construct was introduced at the C-terminus of Acta2 (PubMed=33162897).
CC   Characteristics: Transduced with a lentiviral library consisting of 33 genes, some of which are retained in the immortalized cell line (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5361; ID2.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5362; ID3.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:1919200; Nanog.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:99187; Zfp42.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
CC   Cell type: Alveolar type 2 fibroblast; CL=CL_4028006.
CC   Breed/subspecies: Sprague Dawley; RS=RS:0000681.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_B7UW ! 10-4A
SX   Male
AG   12-14W
CA   Transformed cell line
DT   Created: 23-06-22; Last updated: 19-12-24; Version: 7
//
RX   PubMed=33162897; DOI=10.3389/fphys.2020.567675; PMCID=PMC7582034;
RA   Nemeth J., Schundner A., Quast K., Winkelmann V.E., Frick M.;
RT   "A novel fibroblast reporter cell line for in vitro studies of
RT   pulmonary fibrosis.";
RL   Front. Physiol. 11:567675.1-567675.17(2020).
//