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Cellosaurus U-2 OS-CRISPR-NUP96-Halo clone no.252 (CVCL_B7FI)

[Text version]
Cell line name U-2 OS-CRISPR-NUP96-Halo clone no.252
Accession CVCL_B7FI
Resource Identification Initiative To cite this cell line use: U-2 OS-CRISPR-NUP96-Halo clone no.252 (RRID:CVCL_B7FI)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with a HaloTag (PubMed=31562488).
Transfected with: UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (mutated = HaloTag).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31562488; DOI=10.1038/s41592-019-0574-9
Thevathasan J.V., Kahnwald M., Cieslinski K., Hoess P., Peneti S.K., Reitberger M., Heid D., Kasuba K.C., Hoerner S.J., Li Y.-M., Wu Y.-L., Mund M., Matti U., Pereira P.M., Henriques R., Nijmeijer B., Kueblbeck M., Sabinina V.J., Ellenberg J., Ries J.
Nuclear pores as versatile reference standards for quantitative superresolution microscopy.
Nat. Methods 16:1045-1053(2019)

Cross-references
Cell line collections (Providers) CLS; 300448
Encyclopedic resources Wikidata; Q112930489
Entry history
Entry creation23-Jun-2022
Last entry update02-May-2024
Version number6