Cellosaurus HVRDe008-A-2 (CVCL_B5S4)
| Cell line name | HVRDe008-A-2 |
|---|---|
| Synonyms | GAPDH-Cas9-Puro clone #28; GC9P clone #28 |
| Accession | CVCL_B5S4 |
| Resource Identification Initiative | To cite this cell line use: HVRDe008-A-2 (RRID:CVCL_B5S4) |
| Comments | Characteristics: Using CRISPR/Cas9 a P2A-eSpCas9-IRES-PuroR-pA construct was introduced at the C-terminus of one allele of GAPDH (PubMed=34741877). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_B207 (HUES 8) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=34741877; DOI=10.1016/j.scr.2021.102591 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; HVRDe008-A-2 |
| Biological sample resources | BioSamples; SAMEA10271031 |
| Encyclopedic resources | Wikidata; Q111733467 |
| Entry history | |
| Entry creation | 17-Mar-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 5 |