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Cellosaurus WAe009-A-76 (CVCL_B5QI)

[Text version]
Cell line name WAe009-A-76
Synonyms IMPe010-A-76
Accession CVCL_B5QI
Resource Identification Initiative To cite this cell line use: WAe009-A-76 (RRID:CVCL_B5QI)
Comments From: Research Institute of Molecular Pathology; Vienna; Austria.
Characteristics: Using TALEN a construct containing CAGGS:loxP-STOP-loxp_mCherryNLS-T2A-PuroR was introduced in the AAVS1 safe harbor locus (hPSCreg=WAe009-A-76).
Characteristics: Using CRISPR/Cas9 a construct containing T2A-H2B-Venus-p2A-CreERT was introduced at the C-terminus of BEST1 (hPSCreg=WAe009-A-76).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP).
Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Cross-references
Cell line databases/resources hPSCreg; WAe009-A-76
Biological sample resources BioSamples; SAMEA10839123
Encyclopedic resources Wikidata; Q112041921
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number5