Cellosaurus U2OS DR-GFP (CVCL_B0A7)
| Cell line name | U2OS DR-GFP | ||||||||||||||||||
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| Synonyms | U-2 OS DRGFP | ||||||||||||||||||
| Accession | CVCL_B0A7 | ||||||||||||||||||
| Resource Identification Initiative | To cite this cell line use: U2OS DR-GFP (RRID:CVCL_B0A7) | ||||||||||||||||||
| Comments | Population: Caucasian. Characteristics: Transfected with a DR-GFP reporter construct. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP. Characteristics: Can be used to monitor homologous recombination efficiency using the DR-GFP reporter (ATCC=CRL-3455). Doubling time: ~18 hours (ATCC=CRL-3455). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. | ||||||||||||||||||
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) | ||||||||||||||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
| Hierarchy | Parent: CVCL_0042 (U2OS) | ||||||||||||||||||
| Sex of cell | Female | ||||||||||||||||||
| Age at sampling | 15Y | ||||||||||||||||||
| Category | Cancer cell line | ||||||||||||||||||
| STR profile | Source(s): ATCC=CRL-3455 Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
| Cross-references | |||||||||||||||||||
| Cell line collections (Providers) | ATCC; CRL-3455 | ||||||||||||||||||
| Encyclopedic resources | Wikidata; Q108821411 | ||||||||||||||||||
| Entry history | |||||||||||||||||||
| Entry creation | 23-Sep-2021 | ||||||||||||||||||
| Last entry update | 10-Sep-2024 | ||||||||||||||||||
| Version number | 7 | ||||||||||||||||||