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Cellosaurus CHO-gmt4D-EPO-GnT I (CVCL_A8SQ)

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Cell line name CHO-gmt4D-EPO-GnT I
Synonyms CHO-gmt4D-GnT I EPO; CHO-gmt4D-GnT I; Clone 13-4-1; 13-4-1
Accession CVCL_A8SQ
Resource Identification Initiative To cite this cell line use: CHO-gmt4D-EPO-GnT I (RRID:CVCL_A8SQ)
Comments Characteristics: Transfected with the pEGD vector containing CMV-EPO-IRES-GnTI-IRES-DHFR.
Knockout cell: Method=ZFN; UniProtKB; Q2MH30; Dhfr.
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3415; EPO.
Genetic integration: Method=Transfection; Gene=UniProtKB; Q2MH30; Cricetulus griseus Dhfr.
Genetic integration: Method=Transfection; Gene=UniProtKB; Q924C0; Cricetulus griseus Mgat1.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_A8SP (CHO-gmt4D)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

CLPUB00638
Goh J.S.Y.
Applications of a novel CHO glycosylation mutant.
Thesis PhD (2014); National University of Singapore; Singapore; Singapore

PubMed=24166780; DOI=10.1002/biot.201300301
Goh J.S.Y., Liu Y.-W., Liu H.-F., Chan K.F., Wan C., Teo G., Zhou X.-S., Xie F.-S., Zhang P.-Q., Zhang Y.-X., Song Z.-W.
Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function.
Biotechnol. J. 9:100-109(2014)

Cross-references
Encyclopedic resources Wikidata; Q108820035
Entry history
Entry creation23-Sep-2021
Last entry update19-Dec-2024
Version number8