ID   CHO-gmt4D-EPO-GnT I
AC   CVCL_A8SQ
SY   CHO-gmt4D-GnT I EPO; CHO-gmt4D-GnT I; Clone 13-4-1; 13-4-1
DR   Wikidata; Q108820035
RX   CelloPub=CLPUB00638;
RX   PubMed=24166780;
CC   Characteristics: Transfected with the pEGD vector containing CMV-EPO-IRES-GnTI-IRES-DHFR.
CC   Knockout cell: Method=ZFN; UniProtKB; Q2MH30; Dhfr.
CC   Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3415; EPO.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q2MH30; Cricetulus griseus Dhfr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q924C0; Cricetulus griseus Mgat1.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_A8SP ! CHO-gmt4D
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 23-09-21; Last updated: 19-12-24; Version: 8
//
RX   CelloPub=CLPUB00638;
RA   Goh J.S.Y.;
RT   "Applications of a novel CHO glycosylation mutant.";
RL   Thesis PhD (2014); National University of Singapore; Singapore; Singapore.
//
RX   PubMed=24166780; DOI=10.1002/biot.201300301;
RA   Goh J.S.Y., Liu Y.-W., Liu H.-F., Chan K.F., Wan C., Teo G., Zhou X.-S.,
RA   Xie F.-S., Zhang P.-Q., Zhang Y.-X., Song Z.-W.;
RT   "Highly sialylated recombinant human erythropoietin production in
RT   large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with
RT   restored GnT I function.";
RL   Biotechnol. J. 9:100-109(2014).
//