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Cellosaurus HT29-5M21 (CVCL_A8Q0)

[Text version]
Cell line name HT29-5M21
Accession CVCL_A8Q0
Resource Identification Initiative To cite this cell line use: HT29-5M21 (RRID:CVCL_A8Q0)
Comments Population: Caucasian.
Characteristics: Mucus-secreting clone of HT-29 which forms a monolayer of polarized cells with strong anti-gastric mucin immunoreactivity and mainly expresses MUC5AC and MUC5B. Resistant to methotrexate and sensitive to 5-fluorouracil.
Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): ECACC=15121706

Markers:
AmelogeninX
CSF1PO11,12
D3S135815
D5S81811,12
D7S82010
D8S117910
D13S31711
D16S53911,12
D18S5113
D21S1129
FGA20,22
Penta D11
Penta E14,16
TH016,9
TPOX8,9
vWA17

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Publications

PubMed=9579576; DOI=10.1002/(SICI)1097-0215(19980504)76:3<383::AID-IJC16>3.0.CO;2-C
Lesuffleur T., Violette S., Vasile-Pandrea I., Dussaulx E., Barbat A., Muleris M., Zweibaum A.
Resistance to high concentrations of methotrexate and 5-fluorouracil of differentiated HT-29 colon-cancer cells is restricted to cells of enterocytic phenotype.
Int. J. Cancer 76:383-392(1998)

PubMed=15050369; DOI=10.1016/j.biolcel.2003.12.005
Leteurtre E., Gouyer V., Rousseau K., Moreau O., Barbat A., Swallow D.M., Huet G., Lesuffleur T.
Differential mucin expression in colon carcinoma HT-29 clones with variable resistance to 5-fluorouracil and methotrexate.
Biol. Cell 96:145-151(2004)

Cross-references
Cell line collections (Providers) ECACC; 15121706
Cell line databases/resources cancercelllines; CVCL_A8Q0
Encyclopedic resources Wikidata; Q102114164
Entry history
Entry creation29-Oct-2020
Last entry update02-May-2024
Version number9