Cell line name |
HT29-5M12 |
Accession |
CVCL_A8P9 |
Resource Identification Initiative |
To cite this cell line use: HT29-5M12 (RRID:CVCL_A8P9) |
Comments |
Population: Caucasian. Characteristics: Has an enterocyte-like phenotype. Resistant to both 5-fluorouracil and methotrexate. Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
- Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
|
Disease |
Colon adenocarcinoma (NCIt: C4349) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_0320 (HT-29) |
Sex of cell |
Female |
Age at sampling |
44Y |
Category |
Cancer cell line |
STR profile |
Source(s): ECACC=15121707
Markers: Run an STR similarity search on this cell line |
Publications | PubMed=9579576; DOI=10.1002/(SICI)1097-0215(19980504)76:3<383::AID-IJC16>3.0.CO;2-C Lesuffleur T., Violette S., Vasile-Pandrea I., Dussaulx E., Barbat A., Muleris M., Zweibaum A. Resistance to high concentrations of methotrexate and 5-fluorouracil of differentiated HT-29 colon-cancer cells is restricted to cells of enterocytic phenotype. Int. J. Cancer 76:383-392(1998) PubMed=15050369; DOI=10.1016/j.biolcel.2003.12.005 Leteurtre E., Gouyer V., Rousseau K., Moreau O., Barbat A., Swallow D.M., Huet G., Lesuffleur T. Differential mucin expression in colon carcinoma HT-29 clones with variable resistance to 5-fluorouracil and methotrexate. Biol. Cell 96:145-151(2004) |
Cross-references |
Cell line collections (Providers) |
ECACC; 15121707
|
Cell line databases/resources |
cancercelllines; CVCL_A8P9
|
Encyclopedic resources |
Wikidata; Q102114163
|
Entry history |
Entry creation | 29-Oct-2020 |
Last entry update | 19-Dec-2024 |
Version number | 10 |
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