Cellosaurus HEK-Dual mTLR7 (NF/IL8) (CVCL_A8CW)
| Cell line name | HEK-Dual mTLR7 (NF/IL8) |
|---|---|
| Accession | CVCL_A8CW |
| Resource Identification Initiative | To cite this cell line use: HEK-Dual mTLR7 (NF/IL8) (RRID:CVCL_A8CW) |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia. Knockout cell: Method=Not specified; HGNC; HGNC:6025; CXCL8. Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3. Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5. Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A. Knockout cell: Method=Not specified; HGNC; HGNC:11917; TNFRSF1B. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection; Gene=MGI; MGI:2176882; Tlr7. Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_A8CQ (HEK-Dual Null) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Web pages | https://www.invivogen.com/hek-dual-mtlr7 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107115296 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 9 |