Cellosaurus cell line HEK-Dual Null (CVCL

Cellosaurus HEK-Dual Null (CVCL_A8CQ)

[Text version]

Cell line name

HEK-Dual Null

Synonyms

HEK-NF-KappaB-SEAP/KI-[IL-8]Lucia

Accession

CVCL_A8CQ

Resource Identification Initiative

To cite this cell line use: HEK-Dual Null (RRID:CVCL_A8CQ)

Comments

Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia.
Knockout cell: Method=Not specified; HGNC; HGNC:6025; CXCL8.
Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3.
Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5.
Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A.
Knockout cell: Method=Not specified; HGNC; HGNC:11917; TNFRSF1B.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0045 (HEK293)
Children:

CVCL_A8CR (HEK-Dual hTLR2 (NF/IL8))	CVCL_A8CS (HEK-Dual hTLR3 (NF/IL8))	CVCL_A8CT (HEK-Dual hTLR5 (NF/IL8))
CVCL_A8CU (HEK-Dual hTLR9 (NF/IL8))	CVCL_A8CV (HEK-Dual mTLR4 (NF/IL8))	CVCL_A8CW (HEK-Dual mTLR7 (NF/IL8))

Sex of cell

Female

Age at sampling

Fetus