Cellosaurus 293-Dual hSTING-A162 (CVCL_A8BL)
| Cell line name | 293-Dual hSTING-A162 |
|---|---|
| Accession | CVCL_A8BL |
| Resource Identification Initiative | To cite this cell line use: 293-Dual hSTING-A162 (RRID:CVCL_A8BL) |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). The Lucia luciferase reporter gene expression depends on the activation of the endogenous IFNB1 promoter as the IFNB1 gene has been replaced by Lucia. Knockout cell: Method=Not specified; HGNC; HGNC:5434; IFNB1. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:27962; STING1 (Note=With p.Ser162Ala). Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_A8BK (293-Dual Null) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Web pages | https://www.invivogen.com/293-dual-hsting-a162 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107113187 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 9 |