Cellosaurus 293-Dual Null (CVCL_A8BK)
| Cell line name | 293-Dual Null | ||||
|---|---|---|---|---|---|
| Synonyms | 293-ISG-SEAP/KI-[IFN-beta]Lucia | ||||
| Accession | CVCL_A8BK | ||||
| Resource Identification Initiative | To cite this cell line use: 293-Dual Null (RRID:CVCL_A8BK) | ||||
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). The Lucia luciferase reporter gene expression depends on the activation of the endogenous IFNB1 promoter as the IFNB1 gene has been replaced by Lucia. Knockout cell: Method=Not specified; HGNC; HGNC:5434; IFNB1. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. | ||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||
| Hierarchy | Parent: CVCL_0045 (HEK293) Children:
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| Sex of cell | Female | ||||
| Age at sampling | Fetus | ||||
| Category | Transformed cell line | ||||
| Web pages | https://www.invivogen.com/293-dual-null | ||||
| Cross-references | |||||
| Encyclopedic resources | Wikidata; Q107113194 | ||||
| Entry history | |||||
| Entry creation | 20-May-2021 | ||||
| Last entry update | 19-Dec-2024 | ||||
| Version number | 9 | ||||