Cellosaurus HeLa/Fucci(CA)5 (CVCL_A7AU)
| Cell line name | HeLa/Fucci(CA)5 |
|---|---|
| Synonyms | HeLa/Fucci(CA)5 #16; HeLa/Fucci(CA)5#16 |
| Accession | CVCL_A7AU |
| Resource Identification Initiative | To cite this cell line use: HeLa/Fucci(CA)5 (RRID:CVCL_A7AU) |
| Comments | Population: African American. Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA5) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct. Characteristics: Transfected with the Fucci(CA)5 probe which consist of the fusion of AzaleaB5 to the ubiquitination domain of human CDT1 (AA 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of h2-3 to the ubiquitination domain of human geminin (AA 1-110). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del). Genetic integration: Method=Transfection; Gene=FPbase; 886AH; AzaleaB5 (Note=Red fluorescent protein from Montipora monasteriata). Genetic integration: Method=Transfection; Gene=FPbase; YQ25V; h2-3 (Note=Bright green/yellow fluorescent protein from Ricordea sp. FP2-3). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0058 (HeLa S3) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Publications | DOI=10.1101/2020.03.30.015156 |
| Cross-references | |
| Cell line collections (Providers) | RCB; RCB4919 |
| Encyclopedic resources | Wikidata; Q107115324 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 8 |