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Cellosaurus UM-UC-3 LLWO2F (CVCL_A5WT)

[Text version]
Cell line name UM-UC-3 LLWO2F
Synonyms LLWO2F
Accession CVCL_A5WT
Resource Identification Initiative To cite this cell line use: UM-UC-3 LLWO2F (RRID:CVCL_A5WT)
Comments Population: Caucasian.
Characteristics: Expresses PPP1CA induced by doxycycline. Transfected with the plasmid 6-HA-PP1alpha-pTEP4m plasmid that express a 6His-HA tagged PP1C1 undert the control of a Teto/CMV promoter.
Characteristics: Transfected with the plasmid pUHD172-1neo which contains a rtTA under the control of the CMV promoter. The reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=12914669).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 9281; PPP1CA (Note=With a N-terminal His6-HA tag).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 795; ATM; Simple; p.Gln2800fs*6 (c.8400delG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000012578; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 14446; PARD3B; Unexplicit; Ex9-18del; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Phe113Cys (c.338T>G); ClinVar=VCV000634690; Zygosity=Unspecified (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A5WS (UM-UC-3 LLWO1)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=12914669; DOI=10.1186/1475-2867-3-12; PMCID=PMC183861
Reeder J.E., Sowden M.P., Messing E.M., Klover P., Villa-Moruzzi E., Ludlow J.W.
Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line.
Cancer Cell Int. 3:12.1-12.12(2003)

Cross-references
Cell line databases/resources cancercelllines; CVCL_A5WT
Encyclopedic resources Wikidata; Q107117258
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number8