Cellosaurus cell line UM-UC-3 LLWO1 (CVCL

Cellosaurus UM-UC-3 LLWO1 (CVCL_A5WS)

[Text version]

Cell line name

UM-UC-3 LLWO1

Synonyms

LLWO1

Accession

CVCL_A5WS

Resource Identification Initiative

To cite this cell line use: UM-UC-3 LLWO1 (RRID:CVCL_A5WS)

Comments

Population: Caucasian.
Characteristics: Transfected with the plasmid pUHD172-1neo which contains a rtTA under the control of the CMV promoter. The reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=12914669).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.

Sequence variations

Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 795; ATM; Simple; p.Gln2800fs*6 (c.8400delG); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000012578; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 14446; PARD3B; Unexplicit; Ex9-18del; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Phe113Cys (c.338T>G); ClinVar=VCV000634690; Zygosity=Unspecified (from parent cell line).

Disease

Bladder carcinoma (NCIt: C4912)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_1783 (UM-UC-3)
Children:

CVCL_A5WT (UM-UC-3 LLWO2F)

Sex of cell

Male

Age at sampling

Age unspecified