Cellosaurus SAM iPSC clone 1 (CVCL_A4GX)
| Cell line name | SAM iPSC clone 1 |
|---|---|
| Accession | CVCL_A4GX |
| Resource Identification Initiative | To cite this cell line use: SAM iPSC clone 1 (RRID:CVCL_A4GX) |
| Comments | Characteristics: Stably expresses dCas9-VP64, a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and EF1a-MS2-P65-HSF1-2A-HygR where the coat protein of MS2 fused to the transactivation domain of p65(RELA) and of heat shock factor 1 (HSF1) (PubMed=27934604). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:9955; RELA. Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9). Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067. Cell type: Fibroblast of skin; CL=CL_0002620. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Originate from same individual | CVCL_A4GY ! SAM iPSC clone 2 |
| Sex of cell | Female |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=27934604; DOI=10.1016/j.scr.2016.10.011 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q105510959 |
| Entry history | |
| Entry creation | 12-Jan-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |