ID   SAM iPSC clone 1
AC   CVCL_A4GX
DR   Wikidata; Q105510959
RX   PubMed=27934604;
CC   Characteristics: Stably expresses dCas9-VP64, a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and EF1a-MS2-P65-HSF1-2A-HygR where the coat protein of MS2 fused to the transactivation domain of p65(RELA) and of heat shock factor 1 (HSF1) (PubMed=27934604).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:9955; RELA.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
OI   CVCL_A4GY ! SAM iPSC clone 2
SX   Female
CA   Induced pluripotent stem cell
DT   Created: 12-01-21; Last updated: 19-12-24; Version: 10
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RX   PubMed=27934604; DOI=10.1016/j.scr.2016.10.011;
RA   Xiong K., Zhou Y., Hyttel P., Bolund L., Freude K.K., Luo Y.-L.;
RT   "Generation of induced pluripotent stem cells (iPSCs) stably
RT   expressing CRISPR-based synergistic activation mediator (SAM).";
RL   Stem Cell Res. 17:665-669(2016).
//