Cellosaurus H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (CVCL_A1HB)
| Cell line name | H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 |
|---|---|
| Synonyms | EFNB2-tdTomato/EPHB4-EGFP dual reporter |
| Accession | CVCL_A1HB |
| Resource Identification Initiative | To cite this cell line use: H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (RRID:CVCL_A1HB) |
| Comments | Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (from parent cell line). Characteristics: Using CRISPR/Cas9 a 2A-eGFP-PuroR cassette was integrated in front of the stop codon of one allele of EPHB4 (PubMed=28696312). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_A1HA (H1 EFNB2-tdTomato clone 14) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=28696312; DOI=10.1073/pnas.1702295114; PMCID=PMC5544294 |
| Cross-references | |
| Cell line collections (Providers) | WiCell; efnb2-tdtomato-ephb4-egfp |
| Encyclopedic resources | Wikidata; Q127380968 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |