Cellosaurus H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (CVCL_A1HB)
Cell line name | H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 |
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Synonyms | EFNB2-tdTomato/EPHB4-EGFP dual reporter |
Accession | CVCL_A1HB |
Resource Identification Initiative | To cite this cell line use: H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (RRID:CVCL_A1HB) |
Comments | Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (from parent cell line). Characteristics: Using CRISPR/Cas9 a 2A-eGFP-PuroR cassette was integrated in front of the stop codon of one allele of EPHB4 (PubMed=28696312). Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_A1HA (H1 EFNB2-tdTomato clone 14) |
Sex of cell | Male |
Age at sampling | Blastocyst stage |
Category | Embryonic stem cell |
Publications | PubMed=28696312; DOI=10.1073/pnas.1702295114 |
Cross-references | |
Cell line collections (Providers) | WiCell; efnb2-tdtomato-ephb4-egfp |
Entry history | |
Entry creation | 30-Jan-2024 |
Last entry update | 30-Jan-2024 |
Version number | 1 |