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Cellosaurus H1 EFNB2-tdTomato clone 14 (CVCL_A1HA)

[Text version]
Cell line name H1 EFNB2-tdTomato clone 14
Accession CVCL_A1HA
Resource Identification Initiative To cite this cell line use: H1 EFNB2-tdTomato clone 14 (RRID:CVCL_A1HA)
Comments Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (PubMed=28696312).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Children:
CVCL_A1HB (H1 EFNB2-tdTomato/EPHB4-EGFP clone 29)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=28696312; DOI=10.1073/pnas.1702295114; PMCID=PMC5544294
Zhang J., Chu L.-F., Hou Z.-G., Schwartz M.P., Hacker T., Vickerman V., Swanson S., Leng N., Nguyen B.K., Elwell A., Bolin J.M., Brown M.E., Stewart R., Burlingham W.J., Murphy W.L., Thomson J.A.
Functional characterization of human pluripotent stem cell-derived arterial endothelial cells.
Proc. Natl. Acad. Sci. U.S.A. 114:E6072-E6078(2017)

Cross-references
Encyclopedic resources Wikidata; Q127380967
Entry history
Entry creation30-Jan-2024
Last entry update10-Sep-2024
Version number2