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Cell line name |
NCOL-1 |
Synonyms |
Normal COLon-1 |
Accession |
CVCL_8375 |
Resource Identification Initiative |
To cite this cell line use: NCOL-1 (RRID:CVCL_8375) |
Comments |
Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911; PubMed=20143388). Originally thought to originate from a normal colonic mucosa sample. Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00243. Population: Caucasian. Derived from site: Metastatic; Left supraclavicular lymph node; UBERON=UBERON_8480056. |
Sequence variations |
- Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 583; APC; Simple; p.Arg1114Ter (c.3340C>T); ClinVar=VCV000236589; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 583; APC; Simple; p.Met1431fs*42 (c.4289delC) (p.T1430fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 583; APC; Simple; p.Arg2816Gln (c.8447G>A); ClinVar=VCV000419681; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 914; B2M; Simple; p.Leu15Phefs*41 (c.43_44delCT); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6768; SMAD2; Simple; p.Ala292Val (c.875C>T); Zygosity=Heterozygous (from parent cell line).
|
Disease |
Colon adenocarcinoma (NCIt: C4349) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_0399 (LoVo) |
Sex of cell |
Male |
Age at sampling |
56Y |
Category |
Cancer cell line |
STR profile |
Source(s): PubMed=15771911
Markers:Amelogenin | X,Y |
D2S1338 | 17,18 |
D3S1358 | 14,17 |
D8S1179 | 9,10 (PubMed=15771911_clone_b) 10 (PubMed=15771911_clone_a) |
D16S539 | 9,12 |
D18S51 | 13,18 |
D19S433 | 13,15 (PubMed=15771911_clone_b) 14,15 (PubMed=15771911_clone_a) |
D21S11 | 28,29,30.2,31.2 (PubMed=15771911_clone_b) 29,31.2 (PubMed=15771911_clone_a) |
FGA | 17,20 (PubMed=15771911_clone_a) 18,20 (PubMed=15771911_clone_b) |
SE33 | 18.2,19.2,20.2,25.2 (PubMed=15771911_clone_b) 19.2,24.2 (PubMed=15771911_clone_a) |
TH01 | 9.3 |
vWA | 17,18 |
Run an STR similarity search on this cell line |
Web pages |
https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx |
Publications | PubMed=8812628; DOI=10.1006/jsre.1996.0323 Deveney C.W., Rand-Luby L., Rutten M.J., Luttropp C.A., Fowler W.M., Land J., Meichsner Meller C.L., Farahmand M., Sheppard B.C., Crass R.A., Deveney K.E. Establishment of human colonic epithelial cells in long-term culture. J. Surg. Res. 64:161-169(1996) PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023 Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M., Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W. SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1. Cancer Genet. Cytogenet. 158:84-87(2005) PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003 Wenzel U., Daniel H. Reconsidering cell line cross-contamination in NCOL-1. Cancer Genet. Cytogenet. 163:95-96(2005) PubMed=20143388; DOI=10.1002/ijc.25242 Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I. Check your cultures! A list of cross-contaminated or misidentified cell lines. Int. J. Cancer 127:1-8(2010) |
Cross-references |
Cell line databases/resources |
cancercelllines; CVCL_8375
Cell_Model_Passport; SIDM00838
|
Biological sample resources |
BioSample; SAMN03151825
|
Encyclopedic resources |
Wikidata; Q54908245
|
Entry history |
Entry creation | 04-Apr-2012 |
Last entry update | 02-May-2024 |
Version number | 29 |
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