ID   NCOL-1
AC   CVCL_8375
SY   Normal COLon-1
DR   BioSample; SAMN03151825
DR   cancercelllines; CVCL_8375
DR   Cell_Model_Passport; SIDM00838
DR   Wikidata; Q54908245
RX   PubMed=8812628;
RX   PubMed=15771911;
RX   PubMed=16271966;
RX   PubMed=20143388;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911; PubMed=20143388). Originally thought to originate from a normal colonic mucosa sample.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00243.
CC   Population: Caucasian.
CC   Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Arg1114Ter (c.3340C>T); ClinVar=VCV000236589; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Met1431fs*42 (c.4289delC) (p.T1430fs); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Arg2816Gln (c.8447G>A); ClinVar=VCV000419681; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 914; B2M; Simple; p.Leu15Phefs*41 (c.43_44delCT); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6768; SMAD2; Simple; p.Ala292Val (c.875C>T); Zygosity=Heterozygous (from parent cell line).
CC   Derived from site: Metastatic; Left supraclavicular lymph node; UBERON=UBERON_8480056.
ST   Source(s): PubMed=15771911
ST   Amelogenin: X,Y
ST   D16S539: 9,12
ST   D18S51: 13,18
ST   D19S433: 13,15 (PubMed=15771911_clone_b)
ST   D19S433: 14,15 (PubMed=15771911_clone_a)
ST   D21S11: 28,29,30.2,31.2 (PubMed=15771911_clone_b)
ST   D21S11: 29,31.2 (PubMed=15771911_clone_a)
ST   D2S1338: 17,18
ST   D3S1358: 14,17
ST   D8S1179: 9,10 (PubMed=15771911_clone_b)
ST   D8S1179: 10 (PubMed=15771911_clone_a)
ST   FGA: 17,20 (PubMed=15771911_clone_a)
ST   FGA: 18,20 (PubMed=15771911_clone_b)
ST   SE33: 18.2,19.2,20.2,25.2 (PubMed=15771911_clone_b)
ST   SE33: 19.2,24.2 (PubMed=15771911_clone_a)
ST   TH01: 9.3
ST   vWA: 17,18
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0399 ! LoVo
SX   Male
AG   56Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 28
//
RX   PubMed=8812628; DOI=10.1006/jsre.1996.0323;
RA   Deveney C.W., Rand-Luby L., Rutten M.J., Luttropp C.A., Fowler W.M.,
RA   Land J., Meichsner C.L., Farahmand M., Sheppard B.C., Crass R.A.,
RA   Deveney K.E.;
RT   "Establishment of human colonic epithelial cells in long-term
RT   culture.";
RL   J. Surg. Res. 64:161-169(1996).
//
RX   PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023;
RA   Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M.,
RA   Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W.;
RT   "SKY and genetic fingerprinting reveal a cross-contamination of the
RT   putative normal colon epithelial cell line NCOL-1.";
RL   Cancer Genet. Cytogenet. 158:84-87(2005).
//
RX   PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003;
RA   Wenzel U., Daniel H.;
RT   "Reconsidering cell line cross-contamination in NCOL-1.";
RL   Cancer Genet. Cytogenet. 163:95-96(2005).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//