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Cellosaurus LCL 721.221 (CVCL_6263)

[Text version]
Cell line name LCL 721.221
Synonyms 721.221; .221
Accession CVCL_6263
Resource Identification Initiative To cite this cell line use: LCL 721.221 (RRID:CVCL_6263)
Comments Part of: International Histocompatibility Workshop cell lines.
Characteristics: Does not express HLA-A, HLA-B and HLA-C. The absence of class I HLA expression allows for functional analysis of individual classI antigens via transformation with individual HLA genes (Millipore=SCC275).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: NCIt; C44386; Gamma radiation.
Misspelling: NTCC721.221; CCRID=1101HUM-PUMC000517.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: B-cell; CL=CL_0000236.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D327 (LCL 721.184)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
STR profile Source(s): ATCC=CRL-1855; CCRID=1101HUM-PUMC000517; Millipore=SCC275; Technion Genomics Center

Markers:
AmelogeninX
CSF1PO11,12
D1S165612,13
D2S44110,15
D2S133819,24
D3S135814,16
D5S81812
D6S104311,14
D7S82010,11
D8S117910,14
D10S124814,15
D12S39120,24
D13S31711,12
D16S53912
D18S5112,13
D19S43312,14
D21S1128
D22S104515
FGA23
Penta D11,14
Penta E5,12
TH019.3
TPOX8,12
vWA14,17 (ATCC=CRL-1855; CCRID=1101HUM-PUMC000517; Technion Genomics Center)
17 (Millipore=SCC275)

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Web pages https://www.atcc.org/en/support/technical-support/faqs/lcl-721-221-atcc-crl-1855
Publications

PubMed=3257565; DOI=10.1073/pnas.85.1.227; PMCID=PMC279517
Shimizu Y., Geraghty D.E., Koller B.H., Orr H.T., DeMars R.I.
Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.
Proc. Natl. Acad. Sci. U.S.A. 85:227-231(1988)

PubMed=2785140; DOI=10.4049/jimmunol.142.9.3320
Shimizu Y., DeMars R.I.
Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line.
J. Immunol. 142:3320-3328(1989)

Cross-references
Cell line collections (Providers) ATCC; CRL-1855 - Discontinued
Millipore; SCC275
Cell line databases/resources CCRID; 1101HUM-PUMC000517
IHW; IHW00001
Lonza; 176
TOKU-E; 4103
Biological sample resources BioSample; SAMN03471255
Encyclopedic resources Wikidata; Q54902215
Experimental variables resources EFO; EFO_0022622
Proteomic databases PRIDE; PXD006455
PRIDE; PXD025499
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number26