ID   LCL 721.221
AC   CVCL_6263
SY   721.221; .221
DR   ATCC; CRL-1855
DR   BioSample; SAMN03471255
DR   CCRID; 1101HUM-PUMC000517
DR   IHW; IHW00001
DR   Lonza; 176
DR   Millipore; SCC275
DR   PRIDE; PXD006455
DR   PRIDE; PXD025499
DR   TOKU-E; 4103
DR   Wikidata; Q54902215
RX   PubMed=2785140;
RX   PubMed=3257565;
WW   https://www.atcc.org/en/support/technical-support/faqs/lcl-721-221-atcc-crl-1855
CC   Part of: International Histocompatibility Workshop cell lines.
CC   Characteristics: Does not express HLA-A, HLA-B and HLA-C. The absence of class I HLA expression allows for functional analysis of individual classI antigens via transformation with individual HLA genes (Millipore=SCC275).
CC   Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
CC   Transformant: NCIt; C44386; Gamma radiation.
CC   Misspelling: NTCC721.221; CCRID=1101HUM-PUMC000517.
CC   Discontinued: ATCC; CRL-1855; true.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: B-cell; CL=CL_0000236.
ST   Source(s): ATCC; CCRID; Millipore
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D12S391: 20,24
ST   D13S317: 11,12
ST   D16S539: 12
ST   D18S51: 12,13
ST   D19S433: 12,14
ST   D21S11: 28
ST   D2S1338: 19,24
ST   D3S1358: 14,16
ST   D5S818: 12
ST   D6S1043: 11,14
ST   D7S820: 10,11
ST   D8S1179: 10,14
ST   FGA: 23
ST   Penta D: 11,14
ST   Penta E: 5,12
ST   TH01: 9.3
ST   TPOX: 8,12
ST   vWA: 14,17 (ATCC; CCRID)
ST   vWA: 17 (Millipore)
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D327 ! LCL 721.184
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 24
//
RX   PubMed=2785140; DOI=10.4049/jimmunol.142.9.3320;
RA   Shimizu Y., DeMars R.I.;
RT   "Production of human cells expressing individual transferred
RT   HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line.";
RL   J. Immunol. 142:3320-3328(1989).
//
RX   PubMed=3257565; DOI=10.1073/pnas.85.1.227;
RA   Shimizu Y., Geraghty D.E., Koller B.H., Orr H.T., DeMars R.I.;
RT   "Transfer and expression of three cloned human non-HLA-A,B,C class I
RT   major histocompatibility complex genes in mutant lymphoblastoid
RT   cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:227-231(1988).
//