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Cellosaurus HT29-18-N2 (CVCL_5942)

[Text version]
Cell line name HT29-18-N2
Synonyms HT29-18N2; HT-29-18 N2; N2
Accession CVCL_5942
Resource Identification Initiative To cite this cell line use: HT29-18-N2 (RRID:CVCL_5942)
Comments Population: Caucasian.
Characteristics: Mucus-secreting clone of parent cell line. When grown in the absence of glucose they form homogeneous epithelial monolayers of columnar cells with typical goblet cell morphology (PubMed=3360261).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_5940 (HT29-18)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
Publications

PubMed=3611191; DOI=10.1083/jcb.105.1.345; PMCID=PMC2114933
Huet C., Sahuquillo-Merino C., Coudrier E., Louvard D.
Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation.
J. Cell Biol. 105:345-357(1987)

PubMed=3360261; DOI=10.1016/0016-5085(88)90678-6
Phillips T.E., Huet C., Bilbo P.R., Podolsky D.K., Louvard D., Neutra M.R.
Human intestinal goblet cells in monolayer culture: characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line.
Gastroenterology 94:1390-1403(1988)

PubMed=2154122; DOI=10.1152/ajpgi.1990.258.1.G96
Lencer W.I., Reinhart F.D., Neutra M.R.
Interaction of cholera toxin with cloned human goblet cells in monolayer culture.
Am. J. Physiol. 258:G96-G102(1990)

PubMed=8589882; DOI=10.1007/BF02634251
Phillips T.E., Ramos R., Duncan S.L.
HT29-18N2 differentiation in a protein-free medium.
In Vitro Cell. Dev. Biol. Anim. 31:421-423(1995)

PubMed=12388101; DOI=10.1152/ajpcell.00010.2002
Steinbrecher K.A., Rudolph J.A., Luo G.-J., Cohen M.B.
Coordinate upregulation of guanylin and uroguanylin expression by hypertonicity in HT29-18-N2 cells.
Am. J. Physiol. Cell Physiol. 283:C1729-C1737(2002)

Cross-references
Cell line databases/resources cancercelllines; CVCL_5942
Encyclopedic resources Wikidata; Q54896589
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number24