Cell line name |
HT29-18-N2 |
Synonyms |
HT29-18N2; HT-29-18 N2; N2 |
Accession |
CVCL_5942 |
Resource Identification Initiative |
To cite this cell line use: HT29-18-N2 (RRID:CVCL_5942) |
Comments |
Population: Caucasian. Characteristics: Mucus-secreting clone of parent cell line. When grown in the absence of glucose they form homogeneous epithelial monolayers of columnar cells with typical goblet cell morphology (PubMed=3360261). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
- Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
|
Disease |
Colon adenocarcinoma (NCIt: C4349) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_5940 (HT29-18) |
Sex of cell |
Female |
Age at sampling |
44Y |
Category |
Cancer cell line |
Publications | PubMed=3611191; DOI=10.1083/jcb.105.1.345; PMCID=PMC2114933 Huet C., Sahuquillo-Merino C., Coudrier E., Louvard D. Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation. J. Cell Biol. 105:345-357(1987) PubMed=3360261; DOI=10.1016/0016-5085(88)90678-6 Phillips T.E., Huet C., Bilbo P.R., Podolsky D.K., Louvard D., Neutra M.R. Human intestinal goblet cells in monolayer culture: characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line. Gastroenterology 94:1390-1403(1988) PubMed=2154122; DOI=10.1152/ajpgi.1990.258.1.G96 Lencer W.I., Reinhart F.D., Neutra M.R. Interaction of cholera toxin with cloned human goblet cells in monolayer culture. Am. J. Physiol. 258:G96-G102(1990) PubMed=8589882; DOI=10.1007/BF02634251 Phillips T.E., Ramos R., Duncan S.L. HT29-18N2 differentiation in a protein-free medium. In Vitro Cell. Dev. Biol. Anim. 31:421-423(1995) PubMed=12388101; DOI=10.1152/ajpcell.00010.2002 Steinbrecher K.A., Rudolph J.A., Luo G.-J., Cohen M.B. Coordinate upregulation of guanylin and uroguanylin expression by hypertonicity in HT29-18-N2 cells. Am. J. Physiol. Cell Physiol. 283:C1729-C1737(2002) |
Cross-references |
Cell line databases/resources |
cancercelllines; CVCL_5942
|
Encyclopedic resources |
Wikidata; Q54896589
|
Entry history |
Entry creation | 04-Apr-2012 |
Last entry update | 05-Oct-2023 |
Version number | 23 |
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